EFFECTS OF MANGIFERIN ON SERUM INFLAMMATORY CYTOKINES, OXIDATIVE STRESS, ISLET CELL APOPTOSIS AND GLUCOSE AND LIPID METABOLISM IN RATS WITH GESTATIONAL DIABETES MELLITUS

Long Chen*, Wanze Xiao**, Man Wang***, #

*Department of Endocrinology, Hanchuan People’s Hospital, Hanchuan, PR China - **Department of Endocrinology, Hubei Traditional Chinese Medicine Hospital, Wuhan, PR China - 3Department of Reproduction, Hanchuan People’s Hospital, Hanchuan, PR China

Objective: To observe the effects of mangiferin on the inflammatory response, oxidative stress, islet cell apoptosis and glucose–lipid metabolism in gestational diabetes rats.

Method: Twenty rats with gestational diabetes mellitus were successfully established and divided into disease model group and treatment group. The healthy group consisted of 10 healthy pregnant rats. Rats were treated intraperitoneally with mangiferin. The levels of CRP, TNF-α, IL-6, ROS, SOD, blood glucose, TC, HDL-C and LDL-C as well as the apoptosis rate of islet cells in the three groups were compared.

Results: The levels of inflammatory cytokines in the three groups increased successively from the healthy group, the treatment group and the disease model group (P<0.05). The ROS levels of the three groups of rats were successively increased from the healthy group, the treatment group and the disease model group (P<0.05). In contrast, the SOD level decreased successively from healthy group, treatment group to disease model group (P<0.05). The apoptosis rate of islet cells in the three groups showed an increasing trend from the healthy group, the treatment group to the disease model group (P<0.05). Observation by apoptosis staining sections showed that the number of islet cell apoptosis was the highest in the disease model, followed by the treatment group and the least in the healthy group. Fasting blood glucose before and after treatment in the disease group was higher than that in the healthy group (P<0.05). Before treatment, fasting glucose levels were comparable between the disease model and the treatment group (P>0.05). Fasting blood glucose levels at two time points after treatment were significantly lower in the treatment group than in the disease model group (P<0.05). TC and LDL-C levels in rats were successively increased from healthy group, treatment group to disease group (P<0.05), while HDL-C levels were successively decreased (P<0.05).

Conclusion: Mangiferin can inhibit the release of inflammatory factors and oxidative stress in rats with gestational diabetes mellitus, reduce the rate of islet cell apoptosis and improve the glucose and lipid metabolism, thereby improving the condition of rats with gestational diabetes mellitus.