EFFECTS OF DIAMMONIUM GLYCYRRHIZINATE ON INFLAMMATORY REACTIONS OF BASAL ARTERIAL WALL IN INTRACRANIAL ANEURYSM AND ITS MOLECULAR MECHANISM

Bin Liu, Yang Zhou, Wei Zheng, Xiaopeng Zhu, Xukai Liu, Deqing Han#

Department of Neurosurgery, Zhuzhou Central Hospital, Zhuzhou, PR China

Objective: To analyze the effects of diammonium glycyrrhizinate (DG) on inflammatory reactions of the basilar arterial wall in intracranial aneurysms and its molecular mechanism. 

Methods: Eighty white rabbits from New Zealand were randomly divided into 4 groups: control, IA (aneurysm modelling without drug intervention), DG (aneurysm modelling intervened by drugs) and NS (aneurysm modelling without normal saline intervention) groups, each with 20 cases marked. White modelling conditions were observed using Masson staining. Expressions of MMP-9 and NF-ΚB in the apex of the basilar artery were assayed using immunohistochemical staining; expressions of MCP-1 and TNF-α of tissue in the apex of the basal artery were detected using an ELISA method. 

Results: Masson staining results showed injurious changes of endarterium and internal elastic force layer in the apex of the arterial wall in various modelling groups, and the animal modelling was successful. After modelling, the middle membrane of the basal artery became thinner, and the differences between DG, IA and NS groups had statistical significance (P<0.05); ELISA detection methods showed that expressions of MCP-1 and TNF-α in the DG group were clearly lower than those of IA and NS groups (P<0.05); immunohistochemical staining methods showed that MMP-9 mostly gathered in middle and external layers, whereas NF-ΚB mostly gathered in the tunica intima of the artery. After modelling, expressions of MMP-9 and NF-ΚB in IA, DG and NS groups were all significantly higher than those of the control group (P<0.05 or 0.01); the expression percentage of MMP-9 and NF-ΚB in the DG group were clearly lower than those of IA and NS groups (P<0.05 or 0.01). 

Conclusion: DG can lower the inflammatory reactions of intracranial aneurysm in white rabbits, inhibiting the occurrence and development of intracranial aneurysm-its mechanism may be related to inhibition of the NF-KB relevant inflammatory reaction.