DANGGUI SHAOYAO SAN EXERTS ANTI-INFLAMMATORY AND ANTI-OXIDENT EFFECTS TO ALLEVIATES DIABETIC NEPHROPATHY

Li Xiaobing*, Hou Luming**, Sheng Guangyu**, You Haixin**, Zhang Xiyao**, Chen Yan**, Cai Ruijuan***, Chen Wenyu**, Miao Xinyan**

*Department of Chinese Integrative Basic Medicine,College of Basic Medicine,Henan University of Traditional Chinese Medicine, Zhengzhou, China - **The Second school of clinical medicine of Henan University of Traditional Chinese Medicine, Zhengzhou, China - ***The First school of clinical medicine of Henan University of Traditional Chinese Medicine, Zhengzhou, China

Objective:This study was carried out to assess the antioxidant and anti-inflammatory  of  Danggui Shaoyao San  on streptozotocin (STZ)-induced diabetic rats. 

Methods: Rats were injected with STZ to obtain diabetes models.Rats were divided into 6 groups:

Normal control group(con), Diabetes model group(model), Danggui Shaoyao San high-dose group (DSS-H:21g/kg/day),Danggui Shaoyao San medium-dose group(DSS-M :14g/kg/day),Danggui Shaoyao San low-dose group (DSS-L:7g/kg/day),Tripterygium wilfordii group(TP).We measured blood glucose , renal injury indexes:urine microalbumin (Alb), β2 microglobulin (β2-MG), renal histopathology ,oxidative stress indicators in kidney: malondialdehyde (MDA) and glutathione peroxidase GSH-Px and inflammatory indexes: interleukin 12(IL-12), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ),Interleukin 4(IL-4) and Interleukin 10(IL-10).

Results: DSS improves renal damage in DN rats as indicated from blood glucose,Alb and β2-MG. Besides, DSS treatment reduced the renal MDA content and raised the renal GSH-Px level in DN rats. And pro-inflammatory factors IL-12, TNF-α, IFN-γ were inhibited by DSS treatment, simultaneously anti-inflammatory factors IL-4, IL-10 were promoted in DN rats.

Conclusion: We speculate that DSS exerts anti-inflammatory and anti-oxidant effects to alleviate kidney damage in diabetic rats