CORRELATION BETWEEN SERUM TPO LEVEL AND PERIPHERAL BLOOD T CELL SUBSETS RATIO AND RELATED CYTOKINES IN PATIENTS WITH APLASTIC ANEMIA

Xingxin Li1, Dan Chen2, Anmao Li3, Li Zou4,*

1Department of Medical, The Sixth People's Hospital of Chongqing, Chongqing, PR China - 2Department of Nephrology, The Sixth People's Hospital of Chongqing, Chongqing, PR China - 3The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China - 4Department of Pharmacy, The Sixth People's Hospital of Chongqing, Chongqing, PR China

Objective: To analyze the correlation between the level of serum thrombopoietin (TPO) and peripheral blood T cell subsets ratio and related cytokines in patients with aplastic anemia.

Methods: A total of 93 patients with aplastic anemia treated in the hematology department of our hospital from September 2017 to January 2019 were randomly selected as the study group. According to the clinical manifestation of patients with different characteristics of blood and bone marrow images, the 93 patients were divided into a chronic aplastic anemia group and an acute aplastic anemia group. There were 48 cases in the chronic aplastic anemia group and 45 cases in the acute aplastic anemia group. At the same time, 50 normal healthy persons, all of whom were examined in our hospital during this period, were selected as the normal control group. The levels of serum TPO, erythropoietin (EPO), T lymphocyte subsets (CD4+, CD8+, CD3+, CD4+/CD8+) and related factors (interleukin-4 (IL-4)), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ) and nitric oxide (NO) were observed, and the correlation between the above indices was analysed.

Results: Compared with the normal control group, the serum TPO and EPO levels in the acute aplastic anemia group and the chronic aplastic anemia group were significantly increased, and serum TPO and EPO levels in the acute aplastic anemia group were markedly higher than those in the chronic aplastic anemia group (P<0.01). The levels of CD4+ and CD4+/CD8+ in the acute aplastic anemia group and the chronic aplastic anemia group were significantly lower than those in the normal control group (P<0.01), and the CD8+ and Th1/Th2 ratio were remarkably higher than those of the control group (P<0.01). The level of CD3+ in the acute aplastic anemia group and the chronic aplastic anemia group was lower than that in the normal control group, but the difference was not statistically significant (P>0.05). Compared with the normal control group, the levels of IL-4, TNF-α and INF-γ in the acute aplastic anemia group and the chronic aplastic anemia group were significantly enhanced, and the levels of each index in the chronic aplastic anemia group were significantly lower than those in the acute aplastic anemia group (P<0.01). The Spearson correlation test showed that serum TPO levels were negatively correlated with CD4+, CD3+ and CD4+/CD8+ and positively correlated with CD8+, IL-4, TNF-α and INF-γ levels.

Conclusion: The level of serum TPO was significantly increased in patients with aplastic anemia; T lymphocyte subsets ratio and the level of relevant cytokines were changed to different degrees, and there was a correlation between the indices.