3D-AMSCS ENHANCE ADIPOSE-DERIVED MESENCHYMAL STEM CELL PROPERTIES AND INCREASE TREATMENT EFFECT

Li Ai*, Jinsong Zhang**, Bing Hai*,#, Yu Cao*, Yanshan Pu*

*Department of Pulmonary and Critical Care Medicine, The 2nd Affiliated Hospital of Kunming Medical University, Kunming, China - **Department of Urology, The 2nd Affiliated Hospital of Kunming Medical University, Kunming, China

Mesenchymal stromal cells (MSCs) are useful for cell therapy because of their potential for multilineage differentiation. In this study, we found that the adipose-derived mesenchymal stem cells (AMSCs) similar to typical MSCs show multipotent differentiation potential to form osteoblasts and adipocytes and are positive for the mesenchymal stem cell marker CD29. AMSCs were prepared according to the conventional (2D) or hanging drop culture (3D) method in the optimum culture conditions. In contrast to those prepared with the conventional method, 3D-AMSCs showed significantly increased osteogenic and adipogenic differentiation ability and stemness of stem cells. When 3D culture was performed in the presence of bleomycin, AMSCs had a better resistance to bleomycin-induced injury. The 3D technique was beneficial for AMSC maintenance and differentiation. Through the mice model experiment, we found that 3D culture results in the enhancement of the therapeutic potential of AMSCs. This optimized culture method can enhance the therapeutic potential of MSCs.