EFFECT OF TEMPERATURE AND SIX STORAGE MEDIA ON HUMAN DENTAL PULP CELLS

FUBO CHEN, SHENGCAI QI, QIXIANG YANG, XU ZHANG, YUANZHI XU, RAORAO WANG

Department of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, 200072, China

Aim: The viability of human dental pulp cells (hDPCs) can affect the long-term prognosis of replanted avulsed teeth. When imme- diate replantation of an avulsed tooth is not possible, the cells should be incubated in a physiological storage medium instantly to main- tain their biological activity. The ability of different storage media to preserve human periodontal ligament fibroblasts(PDLF) viability has been previously evaluated, Few studies have showed the effect of different storage media and temperature on the viability of to hDPCs in vitro. The aim of this study was to evaluate the efficacy of the storage media and temperature on the viability of hDPCs.

Material and methods: hDPCs which were obtained from extracted immature human teeth were kept at DMEM, Hanks’ buffered salt solution (HBSS), 0.9% saline, saliva, milk (Fresh milk, Bright dairy, China), or tap water (negative control) (n = 5) under 4°C and 37°C for 1, 2, 4, 8 or 24 h, respectively (n = 5). This study was designed to measure hDPCs activity by CCK-8 assay.

Results: At 4°C and 37°C, milk, HBSS, and DMEM were absolutely superior storage media in maintaining the viability of hDPCs than tap water (the negative control), saliva, and saline at every storage period (P < 0.001).

Conclusion: HBSS was the best storage medium, followed by Milk and DMEM. HBSS, Milk and DMEM can be indicated for the conservation of hDPCs up to 4h.