DISTRIBUTION OF RH BLOOD GROUP D, C AND E ANTIGENS AND THEIR APPLICATION IN CLINICAL SAFE DELIVERY

Yongmei Yuan¹, XiaoYang Wu¹, Xian Li¹, QingWei Yang¹, Baona Chen¹, ChangLin Wu²

¹Department of Blood Transfusion, Shajing People’s Hospital of Bao’an Shenzhen, Shenzhen 518104, China - ²Department of Blood Transfusion, Shenzhen second people’s hospital, Shenzhen 518037, China

 Objective: To understand the distribution of the three antigens of the Rh blood group D, C and E in the population, so as to provide the blood same as its own blood type antigen for the patients with different antigens in clinical delivery, and guide the clinical safety of blood.

Methods: The Rh blood group antigen and antibody were detected by micro column gel technique in 16300 patients. The cross blood matching test was carried out on 8 patients with cross matching blood disagreement by polyamine and anti-human globulin card method, and 140 patients who needed blood delivery were divided into experimental group and control group. In the experimental group, the phenotypes of RhC and RhE were the same, and the RhC and RhE phenotypes of the control group were different. The two groups were fed with 2 units of red cell suspension, and the adverse reactions were observed, and the changes of hemoglobin and he- matocrit before and after blood delivery in two groups were recorded, and statistical analysis was carried out.

Results: Among 16300 patients in our hospital, the positive rates of Rh (D), Rh (C) and Rh (E) antigen were 99.74%, 86.66% and 47.03%, respectively. During the observation period, 8 cases of irregular antibody positive cases were found in our hospital, and most of the antibodies were E and C. In 140 patients who needed blood delivery, there was no significant difference in hematocrit and hemoglobin between the experimental group and the control group, that is, P > 0.05. However, the two values of the control group after blood delivery were lower than those of the experimental group, that is, P < 0.05. It was also found that the number of hemoglobinuria, fever and back pain showed in the experimental group showed was lower than that of the control group after the blood delivery, which was statistically significantly less than that of the control group, that is, P < 0.05.

Conclusion: Rh blood group antigen detection and the implementation of the same antigen delivery can avoid the Rh blood sys- tem to produce antibodies, reduce the various adverse reactions caused by clinical delivery, and improve the safety of clinical delivery.