CORRELATION BETWEEN HOTAIR GENE POLYMORPHISM AND RISK OF BLADDER CANCER AND ITS EXPRESSION AND SIGNIFICANCE IN BLADDER CANCER

Dawei Cai¹, Dong Chen¹, Zongjian Liu², ShaoZhong Xian¹, guangqi Kong¹

¹Department of Urology, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China - ²Central Laboratory, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China

 Objective: To explore the intrinsic correlation between HOTAIR gene traits and the risk of bladder cancer, and to clarify the expression of HOTAIR gene in bladder cancer patients.

Method: The experiment was conducted in Beijing Luhe Hospital from August 2006 to April 2008. The blood of the bladder cancer patients and the normal population was collected. On the basis of the extraction of DNA, the genotypes were classified. The genotype frequency distribution of the SNPs (single nucleotide polymorphisms) in the HOTAIR gene in the patients with bladder cancer and the control group was further compared and analyzed. The correlation between the SNPs genotype and the risk of bladder cancer was discussed. Taking the bladder cancer normal tissue as the reference, the expression of HOTAIR RNA in bladder cancer tissue was measured by qRT-PCR test, and the effect of HOTAIR on the survival time of bladder cancer patients and the correlation of prognosis were determined.

Results: Compared with the reference, the rs874945 locus of the HOTAIR gene significantly affected the frequency of the dis- tribution of GG, AG and AA genotypes. Referring to GG gene type, the risk degree of bladder cancer in AG/AA and AG genotypes reached significant level (P<0.05), 1.095 times and 1.113 times as much as GG genotype, indicating that A allele increased the risk of bladder cancer to a certain extent, and there is a strong relationship between the two parts. Compared with normal tissue, the rel- ative expression of HOTAIR in the bladder cancer tissue was significantly increased. The analysis of the expression of HOTAIR and the pathological related indicators showed that the stage and classification of the tumor were the main factors leading to the high expression of HOTAIR gene (P<0.05), and the survival rate of bladder cancer patients with low expression of HOTAIR in five years was significantly improved (P<0.05).

Conclusion: there is a significant correlation between the rs874945 locus in HOTAIR and the risk of bladder cancer, and the low expression of HOTAIR gene can effectively improve the survival rate of bladder cancer patients.