Yingqi Shi#, Jianwei Qiu, Junbo Qian, Yamin Wang, Zhenming Ge
Department of Gastroenterology, Nantong First People’s Hospital, Nantong, PR China
Objective: To investigate the correlation between miRNA-10a expression and serum TNF-α and IL-6 levels in patients with inflammatory bowel disease and its diagnostic value.
Methods: 65 patients with inflammatory bowel disease treated in our hospital from May 2017 to September 2018 were selected as observation group, and other 65 patients without digestive system diseases were selected as control group. The expression of miRNA-10a in intestinal mucosa, serum and peripheral blood mononuclear cells (PBMC), serum tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) levels were detected by fluorescence quantitative PCR (FQ-PCR). Pearson correlation test was used to analyze the correlation between miRNA-10a expression and serum TNF-α and IL-6 levels. ROC curve was used to evaluate the value of miRNA-10a in the diagnosis of inflammatory bowel disease.
Results: Compared with the control group, the relative expression of miRNA-10a in serum and PBMC in UC patients and CD patients in the observation group was significantly lower than that in the control group, and the levels of TNF-α and IL-6 in the serum of UC patients and CD patients in the observation group were significantly higher than those in the control group (P<0.05), and the expression of miRNA-10a was positively correlated with the levels of serum TNF-α and IL-6 (r = 0.538, 0.613, P<0.01). The results of ROC curve showed that the area under the curve of miRNA-10a was 0.891 (95%CI: 0.810~0.971), the sensitivity was 90.8%, and the specificity was 93.3%.
Conclusion: The expression of miRNA-10a in patients with inflammatory bowel disease is significantly higher than that in healthy patients, and is closely related to the levels of TNF-α and IL-6 in serum of inflammatory factors. The sensitivity and specificity of miRNA-10a expression in the diagnosis of IBD are higher. It may be a new target for IBD therapy.
Inflammatory bowel disease, miRNA-10a, serum TNF- α, IL-6, correlation.