Shuiying Wang*, Xin Jin*, Yang Yu*, Yu Sun**, Qingqing Zhao*, Qun Wang*, Yong Liu*,#
*Cancer Institute (Xuzhou) of Southeast University, Jiangsu 221009, China - **Yue-yang Hospital of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
Objective: To explore the role of miR-875-5p in targeting non-small cell lung cancer to promote invasion and proliferation of non-small cell lung cancer cells by targeting SATB2.
Methods: The expression of SATB2, E-cadherin and other protein under the regulation of miR-875-5p detected by WB test. The effect of miR-875-5p on proliferation and invasion of non-small cell lung cancer cells was studied by miR-875-5p mimic, an inhibitor of miR-1209, scratch method and Tranwell assay, respectively. And the expression of miR-875-5p in non-small cell lung cancer cells was detected by Real-time quantitative PCR. The bioinformatics software was used to analyze its possible target and then adopt luciferase reporter gene to detect the regulation of SATB2. Finally, animal experiments were carried out to further characterize whether miR-875-5p affects cell proliferation and invasion of non-small cell lung cancer through SATB2 or not.
Results: The expression of miR-875-5p was increased in human non-small cell lung cancer cells compared with normal human lung cancer cells (P<0.01). Proliferation and invasion ability of non-small cell lung cancer cells was decreased through inhibition of miR-875-5p expression (P<0.05). The expression of SATB2 would be reduced when miR-875-5p binds to the SATB2 3'UTR region.
Conclusion: miR-875-5p acts as a cancer-promoting small RNA that promotes the proliferation and invasion of non-small cell lung cancer cells by inhibiting the expression of SATB2.
non-small cell lung cancer, miR-875-5p, proliferation, invasion, SATB2.