Junqi Wang, Xiaowei Fu, Xiaolei Xue, Shangkun Lei, Chao Zhang, Yong Jia, Haipeng Li, Zhi Yang#
Department of Thoracic Surgery, Baoji Central Hospital, Baoji 721008, China
LncRNAs play key role on the development and progression of multiple carcinomas. Here, we aimed to evaluate lncRNA DUXAP8 expression in esophageal squamous cell carcinoma (ESCC) tissues and explore the relationship between ESCC and lncRNA DUXAP8. Results showed that lncRNA DUXAP8 was remarkably upregulated in ESCC tissues and cells compared with that in paracarcinoma tissue and Het-1 cells. Meanwhile, a higher DUXAP8 expression was correlated with larger tumor size, lower PTEN levels, but higher Ki67 and ERK1/2 levels. Furthermore, the knockdown of DUXAP8 obviously reduced the binding of LSD-1 to PTEN promoter regions. After siPTEN treatment, ESCC cells with DUXAP8 silencing exhibited an obvious decrease in PTEN expression. Importantly, the interference of sh-DUXAP8 effectively suppressed the proliferation, migration and invasion of ESCC cells in a time-dependent manner, however, this inhibitory effect of sh-DUXAP8 could be partly reversed by siPTEN, thus, lncRNA DUXAP8 was overexpressed in ESCC tissues and could promote the progression of ESCC by silencing PTEN. This study suggests that lncRNA DUXAP8 may act as a noncoding oncogene in ESCC tumorigenesis and is a potential cancer biomarker for ESCC diagnosis and gene therapy.
ESCC, lncRNA DUXAP8, PTEN gene, short hairpin RNA interference.