Authors

Xingang Huang*, Yunpeng Yu**, Xiangjun Jiang***,#

Departments

*Department of Pathology, Qingdao Municipal Hospital, No.1, Jiaozhou Road, Qingdao, 266011 - **Department of Emergency, Qingdao Municipal Hospital, No.1, Jiaozhou Road, Qingdao, 266011 - ***Department of Gastroenterology, Qingdao Municipal Hospital, No. 1, Jiaozhou Road, Qingdao, 266011

Abstract

Objective: To investigate the effect of single acylglycerlipase (MAGL) inhibitor JZL184 on apoptosis and metastasis of colorectal cancer cells and its possible mechanism. 

Methods: Human colorectal cancer SW480 tumor cells were cultured, wall attached growth cells were added with 10 mol/L JZL184 as the experimental group, and DMSO medium was added as the control group. Flow cytometry was used to detect the effect of JZL184 on the apoptosis of colorectal cancer tumor cells. Transwell migration assay was used to detect the effect of JZL184 on the migration ability of colorectal cancer SW480 tumor cell lines. Western blotting was used to detect the expression levels of p-AKT and p-mTOR proteins regulated by JZL184 in apoptotic BCL-2 and Bax, EMT related proteins E-cadherin and Snail, and AKT-mTOR pathway.

Results: Flow cytometry showed that compared with the control group, the apoptosis of colorectal cancer SW480 tumor cells in the experimental group was significantly increased (P<0.05). JZL184 significantly inhibited the migration ability of human colorectal cancer SW480 tumor cell lines, and the difference between the experimental group and the control group was statistically significant (P<0.05). The results of Western Blot analysis showed that the apoptosis-related protein BCL-2 in the experimental group was significantly lower than that in the control group, while the Bax protein was significantly higher than that in the control group (P<0.05). Emt-related protein E-cadherin in the experimental group was significantly higher than that in the control group, while Snail protein was significantly lower than that in the control group (P<0.05). p-AKT, and p-mTOR regulatory proteins in colorectal cancer cell lines in the experimental group were significantly lower than those in the control group (P<0.05)

Conclusion: JZL184 can significantly inhibit the apoptosis and metastasis of colorectal cancer cells, and its mechanism may be related to the regulation of AKT-mTOR pathway to regulate related molecules and thereby affect the expression of apoptosis-related proteins BCL-2, Bax and EMT-related proteins.

Keywords

JZL184, colorectal cancer, apoptosis, metastasis.

DOI:

10.19193/0393-6384_2019_6_459