Authors

Kin Pan Leong*,**,***,****,#, Yu Chan*****, ******, Jing Lin*****, ******, Lizhu Chen*****, ******, Hio Lam Leon*******

Departments

*CCIC Group, Kuok Kim (Macao) Medical Center III - **Kuok Kim (Macao) hygieng testing company limited - ***Lovelo Medical Group, Hong Kong - ****Hui Xian Medical Center, Macao - *****Department of Medical Oncology, Fujian Cancer Hospital & Fujian Medical University, Fuzhou Fujian, China - ******Cancer Bio-immunotherapy Center, Fujian Cancer Hospital & Fujian Medical University, Fuzhou Fujian, China - *******College of Pharmacy, Jinan University, Guangzhou, China

Abstract

Objective: To investigate the effects of JZL184 on the proliferation, apoptosis and metastasis of colorectal cancer cells by inhibiting the activity of monoglyceride lipase (MAGL) and the sensitivity of cancer cells to chemotherapeutic drugs. 

Methods: Human colorectal cancer SW480 cells were purchased and cultured. The cells were divided into experimental group (adding 10 μmol/L JZL184 adherent growth cells) and control group (adding DMSO culture medium alone). The cells were cultured and inoculated. 10 μmol/L JZL184 was added into each pore and 5-FU (10 μmol/L, 200 μmol/L) was added at the same time with different concentrations of 5-F at different concentrations. U (10 μmol/L, 200 μmol/L) was used as control. MTT assay was used to detect the effect of JZL184 on the proliferation of colorectal cancer cells, flow cytometry was used to detect the effect of JZL184 on the apoptosis of colorectal cancer cells, and Transwell migration assay was used to detect the effect of JZL184 on the migration of SW480 colorectal cancer cells. 

Results: The inhibition rate of JZL184 combined with 5-FU at different concentrations was significantly higher than that of 5-FU alone (P < 0.05); the results of flow cytometry showed that the apoptotic rate of human colorectal cancer cells in the experimental group was significantly higher than that in the control group (P < 0.05); the apoptotic rate of tumor cells in JZL184 combined with 5-FU at different concentrations (10 μmol/L, 200 μmol/L) was significantly higher than that in the control group (P < 0.05). The number of cells in the experimental group was significantly lower than that in the control group (P < 0.05). 

Conclusion: JZL184 can inhibit the activity of MAGL, enhance the sensitivity of cancer cells to 5-FU, inhibit the proliferation and metastasis of colorectal cancer cells, and promote their apoptosis.

Keywords

JZL184, colorectal cancer, proliferation, apoptosis, metastasis.

DOI:

10.19193/0393-6384_2019_6_458