Yang Yang*, Man-Xia Lu**, Zhi-Peng An*, Hong-Chi Zhang*, Xiang-Qian Liu**#, Eun-Hee Hwang***#
*School of Life Sciences, Datong University, Datong, China - **School of Pharmacy, Hunan University, Changsha, China - ***Department of food and Nutrition, Wonkwang University, Iksan, Korea
bjective: To find out whether the acankoreagenin, from the leaves of Acanthopanax gracilistylus, showed the hypoglycemic effects in streptozotocin (STZ)-induced diabetic rats.
Methods: The diabetic rats were induced by a single intraperitoneal injection with STZ (70 mg/kg). At the end of the experiment the rats were euthanized, and the plasma/serum was separated for the determination of blood glucose, insulin, biochemical parameters. The pancreatic tissue was used for histopathological and immunohistochemical measurement of NF-κB.
Results: Treatments of acankoreagenin (2 mg/kg) group led to a significant reduction in diabetic rats in blood glucose assessed via oral glucose tolerance test (OGTT) at 2 h. The treatment also resulted in HbA1c level (from 12.04% reduced to 7.01%), lipid profile (triglyceride lever was reduced from 145.83 to 74 mg/dl and total Cholesterol level was reduced from 101.33 to 78.66 mg/dl. Through inhibiting NF-κB, the proinflammatory cytokines release was suppressed in STZ-induced diabetic rats. In pancreatic tissues, histopathological injury was also observed.
Conclusion: These findings are in support of the beneficial effect of acankoreagenin treatment in diabetic rats, and we attribute it to its hypoglycemic effects.
Diabetes mellitus, blood glucose, pancreas.