Authors

Xianghong Kong*, Xirong Li**, Chaoyong Yuan**, Zhihua Zhang**, Yan Li**, Liping Zhou**, Ran Huo*,#

Departments

*Department of Burn and Plastic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China - **Department of Burn and Plastic Surgery, Jining 1st People’s Hospital, Jining, Shandong Province, China

Abstract

Objective: To investigate the expression and correlation analysis of phosphatidylinositol 3-kinase (pi3k), protein kinase B (akt) and mammalian target of rapamycin (mtor) in the mtor signalling pathway in patients with pathological scars.

Methods: Thirty-six patients with pathological scars diagnosed in our hospital from April 2014 to January 2017 were divided into a hypertrophic scar group (n=18) and a keloid group (n=18) according to their pathological types. The normal skin tissue of 18 cases of the hypertrophic scar group was selected as a control group. RT-PCR and Western Blot was used to detect the expression of pi3k, akt, mtor, mRNA and protein in normal tissue, hypertrophic scar tissue and keloid tissue. Moreover, the correlation between mRNA of pi3k, akt and mtor in pathological scar was analysed.

Results: The results of RT-PCR showed that the expression of pi3k mRNA in hypertrophic scar tissue and keloid tissue was not significantly different from normal skin tissue (P>0.05). The expression of akt and mtor mRNA in hypertrophic scar tissue and keloid tissue was significantly lower than normal skin tissue (P<0.05). Moreover, the expression of akt and mtor mRNA in hypertrophic scar tissue was significantly higher than keloid tissue (P>0.05). Western Blot results showed that akt and mtor protein bands in hypertrophic scar tissue and keloid tissue were significantly lower than those in normal skin tissue, but pi3k protein bands in hypertrophic scar tissue and keloid tissue were not significantly decreased. The expression levels of akt and mtor protein in hypertrophic scar tissue and keloid tissue was significantly lower than in normal skin tissue, and the difference was statistically significant (P<0.05). However, the expression levels of pi3k protein in hypertrophic scar and keloid tissue was not significantly different from normal skin tissue (P>0.05). The expression levels of akt and mtor protein in hypertrophic scar tissue was significantly higher than keloid tissue. The results of further correlation analysis showed that there was a positive correlation between mRNA expression of pi3k and akt, akt and mtor, and pi3k and mtor in pathological scar tissue.

Conclusion: The expression of mRNA and protein of pi3k, akt and mtor was low in pathological scar tissue. There was a positive correlation between mRNA expression of pi3k and akt, akt and mtor, and pi3k and mtor in pathological scar tissue.

Keywords

Pathological scar, PI3K, AKT, mTOR.

DOI:

10.19193/0393-6384_2019_6_515