Xuejun He*, Licang Zhu*, Xiaofen He**, Pengrui Xing***, Linzhi Dai*, Liqing Zhu***, Jiayu Zhu*, Wei Han****, Jiangtao Dong*, Dong Zhao*#
*Department of Neurosurgery of the First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang 832008, China - **Shihezi Maternity and Infant Hospital, Shihezi, Xinjiang 832000, China - ***the Medical College of Shihezi University, Shihezi, Xinjiang 832008, China - **** Department of Burn of the First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang 832008, China
Background: This study was designed to investigate the role of Cx43 protein in the pathomechanism and treatment of cerebral vasospasm (CVS) after experimental subarachnoid hemorrhage (SAH).
Methods: A total of 24 Sprague–Dawley rats were assigned randomly to four groups: control (n=6), sham (n=6), operation (n=6), and treatment (n=6). The rats were killed on day 7. A two-SAH model of Sprague–Dawley rats was established by injecting autogenous blood into the front pool of the optic chiasma on day 7 of postoperative death. The diameters of basilar arteries were determined through pressure myography. HE staining was performed to identify the neurologic deficit score between the groups of rats. The Cx43 proteins in the basilar arteries of the different groups were examined by Western blot. The electrophysiological changes in gap junction (GJ) channels in the basilar arteries were observed via a whole-cell patch-clamp technique.
Results: The diameter, Cx43 proteins, neurologic deficit score, and conductivity of GJ channels in the basilar arteries were compared between the groups. Significant differences were observed in the operation group compared with the control, sham, and treatment groups (P<0.01). Conversely, the differences between the control, sham, and treatment groups were not significant (P>0.05).
Conclusions: Cx43 proteins play an important role in the pathomechanism of CVS, and 18β-glycyrrhetinic-acid can relieve CVS in rats in vivo.
cerebral vasospasm, Cx43, 18β-Glycyrrhetinic acid, pressure myography, whole-cell patch-clamp technique.