THE EXPRESSIONS OF PINP, β-CTX AND sICAM-1 IN TYPE 2 DIABETES MELLITUS AND MICROVASCULAR DISEASE

Yanqing qi1, XiaoYun Zhang1, TianTian Ren2, Yakun gao1, hongYu Wu1

1Department of Cardiovascular Surgery, Ningbo First Hospital, No. 59, Liu Ting Street, Ningbo 315010 - 2Department of Hand and Foot Surgery, Ningbo First Hospital, No. 59, Liu Ting Street, Ningbo 315010

 Objective: The purpose of this study is to investigate the expressions of PINP, β-CTX and sICAM-1 in Type 2 diabetes mellitus and microvascular disease.

Methods: A total of 315 patients with type 2 diabetes mellitus (T2DM) who were recruited from January 2015 to December 2016 were included in this study. The subjects were randomly assigned to either the control group or the observation group. Each group included patients with or without retinopathy and/or microalbuminuria. The general data of the patients’ height and weight were recorded in detail. An automatic biochemical analyser was used to determine fasting glucose, blood calcium and other biochemical indicators. Parathyroid hormone, glycosylated haemoglobin and other indicators were measured by radioimmunoassay. The levels of PINP, β-CTX and sICAM-1 were determined by an ELISA.

Results: Compared with the control group, the expressions of HbA1C, TC, TG, Cr, BUN, UA, PTH, β-CTX, and sICAM-1 in the observation group were significantly increased (p<0.05), whereas the 25(OH)D3 level was obviously lower (p<0.05). For the DN, DR and DN/DR groups, the levels of PINP and sICAM-1 in the DN/DR group were significantly higher than those in the control group (P<0.01). The levels of β-CTX in the DR and DN/DR groups were significantly higher than those in the control group (P<0.01). The levels of β-CTX and sICAM-1 in the DN group were significantly lower than those in the DN/DR group (P<0.01). In the control group, β-CTX expression was negatively correlated with BUN (γ=-5.586, P=0.003), Cr (γ=-6.159, P=0.000) and UA (γ=-3.087, P=0.038). In the observation group, PINP was negatively correlated with BUN (γ=-0.357, P=0.016) and UA (γ=-0.063, P=0.041). β-CTX was negatively correlated with BUN (γ=-0.456, P=0.008), Cr (γ=-6.122, P=0.000), UA (γ=-5.568, P=0.000) and 25(OH)D3 (γ=-6.897, P=0.000). Moreover, PINP was positively correlated with HbA1C (γ=3.016, P=0.019) and PTH (γ=6.973, P=0.000). sICAM-1 was positively correlated with LDL (γ=0.486, P=0.007).

Conclusions: The β-CTX and sICAM-1 expression levels can be used as predictors to monitor the early risk stratification in T2DM and microvascular disease.