RELATIONSHIP BETWEEN EXPRESSION CASPASE-3 AND APOPTOSIS IN THE HIPPOCAMPUS OF RATS AFTER SUBARACHNOID HEMORRHAGE

LICANG ZHU¹, XUEJUN HE¹, JING DAI¹, LINZHI DAI¹, XIAOFEN HE², JIAN XU¹, JIANGTAO DONG¹, YANG LI¹, DONG ZHAO¹, LI LI³

¹Department of Neurosurgery of the First Affiliated Hospital of the Medical College, Shihezi University, Shihezi, Xinjiang 832008, China - ²Maternal and Child Health Hospital of Shihezi, Xinjiang 832000, China - ³the Medical College of Shihezi University,Shihezi, Xinjiang 832000, China

Introduction: In this experiment, the expression of Caspase-3 and apoptosis of hippocampal neurons was detected, and the rela- tionship between Caspase-3 and apoptosis of neurons after subarachnoid hemorrhage (SAH) was discussed.

Materials and methods: 48 male SD rats were randomly divided into two groups, 6 rats in the blank group, and 42 rats in the experimental groups, The experimental groups were then randomly divided into 1h, 3h, 6h, 12h, 24h, 48h,72h and Each subgroup of 6.

Results: The results of HE staining showed that there were more red blood cells in the subarachnoid space . Subarachnoid hemorrhage groups 1h(16.50±0.55), 3h(15.80±0.41), 6h(15.33±0.52), 12h(14.50±0.55), 24h(13.67±0.52), 48h(14.10±0.41), 72h(15.00±0.63) had lower neurological score and had statistically significant compared with the control group (17.83±0.41) (P< 0.05). The neurological behavior scores of rats in the SAH group showed a trend of first decrease, and the 24h was the lowest, the diffe- rence was statistically significant (P < 0.05). TUNEL results showed that the apoptosis of SAH cells increased at first and then decrea- sed. 1H began to appear in the cell apoptosis, 1H and 3H apoptosis cells were less, 6h increased, and the apoptosis reached the peak at 24. The apoptosis of 48h and 72h decreased gradually, and the difference was statistically significant (P < 0.05). Caspase-3 protein expression: 3h, 6h, 12h, 24h, 48h, 72hSAH, 1H group were significantly higher than the control group, the difference was statistically significant (P < 0.01), 1H group SAH began to rise, 6h rose rapidly, 24 hours to reach the peak, 72h, 48h began to decline, the differen- ce was statistically significant (P < 0.05). There was positive correlation between Caspase-3 and neuronal apoptosis (r=0.730, P < 0.05).

Conclusions: Caspase-3 plays an important role in the pathological process of early brain injury after SAH.