RELEVANCE BETWEEN FGFR - 2 GENE POLYMORPHISM AND BREAST CANCER

YANG WANG^1,2, HAIYU ZHANG³, YONGSHENG WANG^1,4*

¹Shandong Cancer Hospital Affiliated to Shandong University, Jiyan Road No. 440, Jinan, Shandong, 250117, China - ²Department of Galactophore Surgery, Weifang People’s Hospital, Guangwen Street No. 151, Weifang, Shandong , 261041, China - ³Department of Antenatal Diagnosis, Weifang People’s Hospital, Guangwen Street No. 151, Weifang, Shandong , 261041, China - ⁴Shandong Academy of Medical Sciences, Jinan, Shandong 250062, China

Objective: To investigate the relevance between the second intron single nucleotide polymorphism of fibroblast growth fac- tor receptor 2 (FGFR2) gene and the onset of breast cancer in women.

Methods: The 132 cases of female breast cancer (breast cancer group) and 145 normal women (control group) were detect- ed by combining polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with agarose gel elec- trophoresis. The polymorphism distribution of two single nucleotide polymorphisms (rs2981579) and (rs2981582) of the second intron of FGFR2 gene was analyzed statistically.

Results: For FGFR2 gene single nucleotide polymorphism rs2981579, the frequencies of genotype (CC, CT, TT) in breast cancer group and the control group were 31.06%, 43.18%,25.76% and 26.90%, 45.52%,27.58% respectively. The frequencies of C allele in breast cancer group and control group were 68.94% and 75.17% respectively, and the frequencies of T allele in the two groups were 31.06% and 24.83% respectively. There was no statistically significant difference in genotype and allele frequency distribution between the two groups. For FGFR2 gene single nucleotide polymorphism s2981582, the frequencies of genotype (CC, CT, TT) in breast cancer group and the control group were 37.12%, 46.97%, 15.91% and 35.86% .45.52% .18.62% respec- tively. The frequencies of C allele in breast cancer group and control group were 72.73% and 71.72% respectively, and the fre- quencies of T allele were 27.27% and 31.72% respectively. The distribution of genotype frequency and allele frequency were com- pared between the two groups and there was no statistically significant difference (p>0. 05).

Conclusion: There is no significant correlation between the 2 polymorphism sites of FGFR2 intron and breast cancer.