EVALUATION OF TOTAL ANTIOXIDANT (TAS) AND TOTAL OXIDANT (TOS) PARAMETERS IN LIVER, KIDNEY AND BRAIN TISSUES OF RATS WHICH ARE APPLIED JWH-200

İSMAIL ÇETINKAYA*, MÜMIN POLAT**

*Mehmet Akif Ersoy University Health Science Institue Laboratory And Experimental Animals - **Mehmet Akif Ersoy University Faculty of Health Science

Synthetic cannabinoids (SC) have recently announced their name as substances which are misused among young adults. Death is commonly observed, since they cause serious toxicity. These effects often cause central nervous system damage . The end result of the use of synthetic cannabinoids is the circulatory and respiratory system as well as the central nervous system. Sudden changes in respiratory rate, rise in heart beat and expansion in blood vessels are significant symptoms. The aim of this study is to reveal changes in Total Antioxidant Level (TAS) and Total Oxidant Level (TOS) parameters in liver, kidney and brain tissues in order to obtain information about the predicted damages caused by synthetic drugs in tissues and organs. In our study, 20 male Wistar albino rats at least 8 weeks old were used. The rats were divided into two groups, experiment and control group, while the difference between the weights of the rats was adjusted to be statistically insignificant (p = 0.231) by two sample t tests. Experimental group rats were implemented as intra peritoneal the mixture of 0.5 ml / kg Ethanol, 4.5 ml / kg Dextrose, 5 mg / kg JWH-200. No implementation was applied to the control group. Decapitation was performed under general anesthesia 8 hours after this implementation. TAS and TOS parameters were studied by taking and preparing liver, kidney and brains of both groups. Oxidative stress indexes were determined by measuring from the organs taken from the experimental and control groups with a spectrophotometer. The difference between the two groups was considered statistically insignificant (p> 0.05). Liver TOS level was determined higher in the experimental group in comparison with control group. In our study, renal TAS findings were deter- mined lower in the experimental group than in the control group. In our study, oxidative stress is formed because of the high levels of antioxidant levels of kidney. It is thought that the use of synthetic cannabinoids in different systems, depending on the dose, can also affect the urinary system and the kidneys at the same time.