EXPRESSION OF KU PROTEINS IN GLIOMA AND THEIR ASSOCIATION WITH RADIOTHERAPY SENSITIVITY AND PROGNOSIS

YANG JIWEN, CHEN QIANXUE

Department of Neurosurgery, Renmin Hospital, Wuhan University Hubei

Objective: To study the expression of KU 70 and KU80 proteins in glioma tissue and their correlations with radiotherapy sen- sitivity and prognosis.

Methods: Glioma tissue samples (20) from patients exposed to radiotherapy in neurological surgery from January, 2014 to December, 2016 were used in the study. Ten (10) normal cerebral tissue samples served as control. Immunochemical staining was used to determine the expressions of KU70 protein and KU80 protein in glioma tissue and normal cerebral tissue. In addition, glioma nude mice models were divided into blank control group (A group), and groups exposed to different degrees of radiation (Gy) for dif- ferent durations i.e. 2 Gy+24 h group (B group), 2 Gy+48 h (C group), 4 Gy+24 h (D group), 4 Gy+48 h (E group), 8 Gy+24 h (F group) and 8 Gy+48 h (G group). Immunochemical staining was used to determine the expressions of KU 70 and KU80 proteins.

Results: Positive expressions of KU 70 protein and KU80 protein in tumor tissue were 65.0 % and 50.0 %, respectively higher than in normal cerebral tissue (p < 0.05). The expressions of these proteins were positively correlated with recent treatment effects and prognosis (p < 0.05). Results from the animal experiments showed that positive expression of KU protein had positive correla- tion with dose of radiation (p < 0.05), but it had no correlation with exposure time (p > 0.05).

Conclusion: Triptolide plays anti-proliferation role in glioma cells by inhibiting the up-regulation of gene-1 expression in astrocytes.