ISOLATION OF ENDOPHYTIC FUNGI FROM THERMOPSIS LANCEOLATA AND THEIR ANTIOXIDANT ACTIVITY

LIN YANG^1*, XIAO-KAI ZHOU¹, LEI WANG¹, HONG-XIA SHI², XIAO-FENG LIU¹, YONG-GANG WANG¹

¹The Key Lab of Screening, Evaluation and Advanced Processing of TCM and Tibetan Medicine, Gansu Educational Department, School of Life Science and Engineering, Lanzhou University of Technology, 730050, Lanzhou, China - ²Lanzhou Inspection and Quarantine Co., Ltd, No.1 of Yingmen village in Anning District of Lanzhou City, Gansu

Introduction: Plant endophytic fungi resources is nearly a decade of research hot spots, endogenous fungal resources develop- ment and utilization will be fruitful.

Aim: Aim of this study is isolation of endophytic fungi strains from healthy tissues of Thermopsis lanceolata and evaluation of antioxidant activity of fermented extracts of these isolated endophytic fungi strains.

Materials and methods: Root, stem and leaf of Thermopsis lanceolata were used to isolate endophytic fungi strains. Reducing power and DPPH free radical scavenging assays were used to evaluate the antioxidant activity of fermented extracts from isolated endophytic fungi strains from Thermopsis lanceolata. Meanwhile, Total phenolic content (TPC) of fungal extracts was tested by Folin-Ciocalteau reagent based assay.

Results: 16 endophytic fungi strains were isolated by tissue culture from healthy root, stem and leaf of Thermopsis lanceolata; Results of antioxidant activity evaluate indicates that there are 4 samples showed a good reduction force, especially ethyl acetate extract of Tlc-R-7 has the highest reducing power, it’s absorbance value reached 0.528 (ascorbic acid, 0.588). Moreover, ethyl aceta- te extracts had generally higher antioxidant activity than extracts of n-butanol and methanol; Result of DPPH free radical scaven- ging activity showed there are 3 extracts samples showed inhibition rate of more than 90%. 2 extracts showed inhibition rate of more than 85%, among them, Tlc-R-7 ethyl acetate extract has the highest inhibition rate of 93%; The results indicate that the higher the phenol content in the sample, the stronger the antioxidant capacity of the sample; Most of the higher activity extracts of the fungal was concentrated in the roots.

Conclusion: There are abundant endophytic fungi resources in Thermopsis lanceolata, and the endophytic fermentation pro- duct has a good prospect of antioxidant drug development.