RESEARCH OF XINFENG CAPSULE ON PULMONARY FUNCTION BASED ON THE B, T CELL IMMUNITY IN THE RAT MODEL OF ADJUVANT INDUCED ARTHRITIS

WAN LEI, LIU JIAN*, HAUNG CHUANBING

Department of Rheumatology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei (230031), China

Introduction: To observe the effects of Xinfeng Capsule on adjuvant arthritis rat model of pulmonary function, B, T cell immunity.

Materials and methods: 40 rats were randomly divided into normal, model, Tripterygium Glycosides tablet and XFC group. Except the normal group, the other rats right rear paw intradermal injection of Freund’s complete adjuvant induced arthritis in 0.1 mL. Inflammation induced 19 d after administration, the normal group, the model group were given physiological saline, rats in the treatment group were given of Xinfeng Capsule (2.4 g/kg), Tripterygium Glycosides tablet (10 mg/kg), once a day, continuous administration of 30D. Joint inflammation in rats was observed performance, flow cytometry detection of peripheral blood B, T lymphocyte attenuator (BTLA), regulatory T cells (Treg) and surface markers of forkhead transcription factor (FoxP3) expression was detected by Western blotting, synovial membrane, lung tissue BTLA, FoxP3.

Results: Compared with the normal group, model group of rat paw swelling degree, arthritis index increased; pulmonary function, reduce the expression levels of peripheral blood BTLA, Tregs and BTLA in pulmonary tissue, synovium, FoxP3 (P <0.05 or P <0.01). While the XFC group rat paw swelling and arthritis index lower than the model group, pulmonary function, BTLA, Tregs and FoxP3 increased (P <0.05 or P <0.01); Xinfeng capsule group is better than control group (P <0.05) medicine Tripterygium wilfordii.

Discussion: Xinfeng capsule may be through upregulation of BTLA, Tregs, FoxP3 expression, reduce the stimulation of inflammatory mediators in the lung tissue and improve the lung function of rats with adjuvant arthritis.