PHARMACOKINETICS STUDY OF AVANAFIL AND ITS ANALOGUES IN RATS

SHUOYE YANG

College of Bioengineering, Henan University of Technology, Zhengzhou, P. R. China

Introduction: dysfunction (ED). The Aim of this study is to investigate the pharmacokinetics properties of avanafil and its analogues.

Materials and methods: After extraction by acetonitrile, the analytes were separated by a rapid gradient elution with acetonitrile and water as the mobile phase and detected by an mass spectrometer. Multiple reaction monitoring was performed on the ion transitions of m/z 484.2→375.2 [M-H] - (avanafil).

Results: A simple, rapid and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of avanafil and its analogues in rat plasma using Dixipan as the internal standard (IS). The plasma concentration of avanafil and its analogues in rats showed good linearity. All the analytes can maintain stable during the whole experimental process.

Discussion: The developed method was successfully applied to the pharmacokinetic study of avanafil and its analogues after oral administration to rats. The results showed that the analogues have better absorption property in vivo than avanafil.