, HATICE SEZEN1
, NURAY ALTAY2
, MEHMET AKIF ALTAY3
, CEMIL ERTÜRK3
, NURTEN AKSOY1
Department of Clinical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey - 2
Department of Anesthesiology
and Reanimation, Faculty of Medicine, Harran University, Sanliurfa, Turkey - 3
Department of Orthopedic Surgery, Faculty of
Medicine, Harran University, Sanliurfa, Turkey
Introduction: Despite the fact that cathepsins (Cat) are known to be associated with the pathogenesis of osteoarthritis (OA),
there is no relevant literature regarding (cystatin-C) Cys-C, which is a natural inhibitor of Cat in human OA. The purpose of this
study is to investigate the levels of Cys-C and total free thiol (TFT) in the serum and synovial liquid of patients with OA.
Materials and methods: Forty OA patients, diagnosed according to the American College of Rheumatology (ACR) criteria,
and 40 healthy individuals were included in the study. In serum and fluid samples taken from patients and controls, the serum TFT
levels were studied with Elman's method, and the Cys-C levels were studied with colorimetric method using commercially available
kits in an autoanalyzer.
Results: Cys-C levels were significantly higher in the patient group than in the control group (p<0.05), and serum TFT levels
were similar between groups (p>0.05). A correlation analysis showed that the levels of serum Cys-C were positively correlated with
the presence of OA (correlation coefficient = 0.300, p = 0.014) and negatively correlated with the TFT levels (correlation coefficient
= -0.231, p = 0.024). In the regression analysis, it was observed that only the presence of OA independently affected the levels of
Cys-C beta regression coefficient = 0.130, p = 0.024).
Conclusion: In patients with OA, the level of serum Cys-C was determined to be higher than in controls. This may be related
with the pathogenesis of disease, increased oxidative stress, or chronic inflammation in OA.
Osteoarthritis, inflammation, Cystatin-C, serum total free thiol