Authors
BERRIN PANCARCI1*#, AHMET UNVER2
, YELIZ ÇETINKOL3
, ONUR ATAKIŞI
4
Departments
1
Department of Microbiology, Institute of Health Sciences, Kafkas University, Kars - 2
Department of Medical Microbiology,
Faculty of Medicine, Canakkale Onsekiz Mart University,Canakkale - 3
Department of Medical Microbiology, Faculty of Medicine,
Ordu University, Ordu - 4
Department of Biochemistry, Faculty of Science and Literature, Kafkas University, Kars, Turkey
Abstract
Introduction: This study aimed to investigate relationships among serum nitric oxide (NO) concentrations, total oxidant-antioxidant
capacities (TOC-TAC), serum C-Reactive Protein (CRP), Aspartate Aminotransferase (AST), Alanin Aminotransferase (ALT) and
hematological parameters in Brucella seropositive (serum samples positive for Brucella) and seronegative (serum samples negative
for Brucella) human serum samples.
Materials and methods: Eighty positive and 20 negative human sera to Brucella Serum Tube Agglutination Test were used for
determination of NO, TOC-TAC, CRP, AST, ALT.
Results: Serum TOC concentrations were significantly (P<0.01) higher in Brucella seropositive titers compared to Brucella
seronegative serum samples regardless of titers. However, there was no difference among different seropositive titers for serum TOC
concentrations. In contrast, no effect of Brucella antibody titers on serum NO, TAC, CRP, AST and ALT levels was proved. However,
AST concentration tended to be (P<0.07) higher at 1/640 titer. While Brucella antibody titers decreased leukocyte (P<0.05) and
increased erythrocyte (P<0.05) counts at 1/640 titers with no change at 1/160 and 1/320 titers, no effect of Brucella antibody titers
was detected on thrombocyte counts. There was a positive correlation between Brucella antibody titers and TOC (r=0,44; P<0,01),
AST (r=0,23; P=0,05) levels and erythrocyte counts (r=0,32; P<0,01); whereas, there was a negative correlation between Brucella
antibody titers and leukocyte counts (r= -0.32; P<0,01).
Conclusion: The increase in TOC levels and steady state levels of TAC could reveal that antioxidant system was efficient during
the course of brucellosis. Furthermore, determination of TOC/TAC system along with hematological and inflammatory parameters
may be helpful monitoring and better understanding the pathogenesis of brucellosis.
Keywords
Brucellosis, nitric oxide, oxidant and antioxidant capacity
DOI:
10.19193/0393-6384_2016_1_10