DUAL SILENCING OF NET-1 AND VEGF GENES AFFECT BIOLOGICAL BEHAVIOR OF NON SMALL CELL LUNG CANCER IN VIVO STUDY

HAI-TAO HUANG¹ , YU QIAO¹ , LI CHEN² , CHUN-QIU XIA¹ , CHONG-JUN ZHONG¹*

¹ Department of thoracic and Cardiovascular Surgery, Nantong first people’s hospital, the Second Affiliated Hospital of Nantong University, Nantong, 226001, P.R. China - ² Department of Pathology, Nantong University, Nantong, 226001, P.R. China

Introduction: To investigate the effects of silencing NET-1 (neuroepithelial cell transforming gene-1) and VEGF (vascular endothelial growth factor) using DGT siRNA (Dual genes targeting small interfering ribonucleic acid) on the biological behavior of lung cancer cell in vivo.

Materials and methods: In this study, 12 nude mice models bearing human lung cancer xenografts were built by injecting A549 lung cancer cell. The targeting NET-1, VEGF siRNA and DGT siRNA embedded by cationic liposome were injected to the xenograft tumor when its volume reached to 100mm3. The expressions of NET-1 and VEGF gene in xenograft tumor were detected by immunohistochemistry. Additionally, proliferation and microvessel density of xenograft tumors were evaluated by detecting the expression of Ki- 67 and CD34 respectively.

Results: Nude mice models bearing xenograft tumor were successfully built. In dual siRNA group, compared with single NET-1 or VEGF siRNAs group, the volume of xenograft tumor was smaller; the expressions of NET-1 and VEGF was lower; the expression of Ki-67 and CD34 was lower as well (P<0.05).

Conclusions: Dual-target genes silencing could achieve better inhibitory effects on the proliferation and the angiogenesis of lung xenograft tumor.