Authors

JING XU1, YI HUANG2, YING XU3, XIAOLIN HUANG3, LI LI3, MIN ZHENG3

Departments

1Comprehensive Breast Health Centre, Zhoushan Hospital, Wenzhou Medical University, Zhoushan, Zhejiang, China - 2Department of Stomatology, Sichuan Provincial People’s Hospital, Chengdu, Sichuan, China - 3Department of Stomatology, Zhoushan Hospital, Wenzhou Medical University, Zhoushan, Zhejiang, China

Abstract

Introduction: To discuss expression characteristics of miRNA-218-5p in invasive frontier cells of oral squamous cell carci- noma (OSCC) and its molecular signal of regulation.

Materials and methods: OSCC invasive frontier cell strains, specifically SCC6-M and SCC6 cancer cell strains, were dis- sociated from cancer tissue and acquired from other parts of tumours of OSCC patients in our hospital. miRNA-218-5p expression level in the two cell strain groups and CD44mRNA expression level in the SCC6-M cell strain after transfection by miRNA-218-5p mimics and inhibitor were compared with a negative control. CD44 protein expression levels of cell strains in two groups were detected using the Western blot method.

Results: Relative expression levels of miRNA-218-5p in the SCC6-M cell strain was lower than that of the SCC6 cell strain (P < 0.05), while relative expression level of CD44 protein in the SCC6-M cell strain was higher than that of the SCC6 cell strain (P < 0.05). Invasion depth, invasion area and CD44mRNA expression level of the SCC6-M cell strain were all lower than those of the negative control group (P < 0.05) 24 h and 48 h after transfection by miRNA-218-5p mimics. Invasion depth, invasion area and CD44mRNA expression level of the SCC6-M cell strain were all higher than those of the negative control group (P < 0.05) 24 h and 48 h after transfection by a miRNA-218-5p inhibitor.

Conclusion: Expression level of miRNA-218-5p in invasive frontier cells of OSCC was lower than that of other parts of the tumour. miRNA-218-5P weakens the CD44/ROCK signal pathway and inhibits tumour metastasis and invasion by inhibiting CD44 protein expression.

Keywords

oral squamous cell carcinoma, invasive frontier cells, miRNA-218-5p, expression, signal pathway.

DOI:

10.19193/0393-6384_2018_6_304