RUNMIN GUO1, HAILIANG MO1, JIAMEI JIANG1, ZIJUN WU1, CHANG LIU1, XUEHUI MENG3, QIONG YOU1, ZHIQIANG WANG2,4, KENG WU1
1Department of Cardiology - 2Clinical Research Center, The Affiliated Hospital, Guangdong Medical University, Zhanjiang 524001, P.R. China - 3Healthcare Reform Research Department of Zhejiang Hospital Development Center, No.216, Qingchun Road, Xiacheng District, Hangzhou City, Zhejiang Province, 310006 PR China - 4Johns Hopkins University, Department of Pediatrics, Baltimore, USA.
Background: This study is aimed at evaluating whether DNA repair protein Apurinic/Apyrimidinic Endonuclease 1 (APE1) correlates with diabetic cardiomyopathy(DCM).
Methods: 32 patients with DCM, 62 DM patients and 60 control subjects were enrolled. 8-OHdG (a marker of oxidative DNA damage) were measured in serum. Expression and DNA repair enzyme activity of APE1 were tested by ELISA kit and modi- fied methods respectively. Cardiac diastolic and systolic function in patients were measured using echocardiography.
Results: The 8-OHdG levels were higher than those of controls. Serum APE1 in DCM patients were elevated and its DNA repair activity was markedly reduced.
Conclusions: Expression and DNA repair enzyme activity of APE1 were closely associated with heart function in DCM patients. Our results suggest that DNA repair deficiency of APE1 might be involved in cardiac injuries under diabetic context.
Diabetic cardiomyopathy, High glucose, Apurinic/Apyrimidinic Endonuclease 1, Apoptosis.