TAO CHEN1, ZHANHUA WANG2
1Gansu province hospital rehabilitation center, Lanzhou 730000, Gansu，China - 2Department of laboratory, Lanzhou Petrochemical General hospital, Lanzhou 730060, Gansu, China
Objective: To investigate the influence of acupoint selection on ghrelin and GHSR expressions in the gastric fundus of rats with diabetic gastroparesis (DGP).
Methods: Sixty male SPF SD rats were given adaptive feeding for one week and were randomly divided into a blank control group, a model group, Zusanli and Zhongwan (ZZ) group, Zusanli and Neiguan group (ZN), as well as Zusanli and non-meridian acupoint (ZNA) group, with 12 rats in each group. Except for the blank group, the other 48 rats were used to establish a DGP model by using streptozotocin. Acupuncture therapy was performed from the end of the tenth week for an additional four weeks. Then the rats were sacrificed and the sera and gastric fundus tissues were collected. Serum ghrelin content was assayed with ELISA kits. The expression of ghrelin positive cells in the gastric fundus tissue was determined by immunohistochemistry, while the expression of ghrelin and GHSR mRNA in the gastric fundus tissue was determined by real-time polymerase chain reaction (RT-PCR).
Results: Compared with the control group, the feed intake and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue in the model group was significantly increased, while small intestinal propulsion rate, serum ghrelin content, and the gray value of the gastric fundus were significantly decreased (p < 0.05). Compared with the model group, the expression of ghrelin and GHSR mRNA in the gastric fundus tissue, small intestinal propulsion rate, serum ghrelin content, and gray value of the gastric fun- dus were significantly increased (p < 0.05). Feed intake was significantly higher in the ZN and the ZNA groups (p < 0.05), and the expressions of ghrelin and GHSR mRNA in the gastric fundus tissue were significantly increased (p < 0.05) but the serum ghrelin content in ZN group was significantly decreased (p < 0.05).
Conclusions: These results suggest that improvement of gastrointestinal motility in DGP by acupuncture therapy is associated with serum ghrelin content and GHSR expression in the gastric fundus. They also indicate that the effect of treatment with local acu- points compatibility is better than that of distal acupoints compatibility, and that acupoint selection according to location or outcome is a key factor that influences acupoints compatibility.
Acupoints compatibility, Acupoints selection, Diabetic gastroparesis, Acupuncture therapy