MEHDI ROSHDI MALEKI1, HOSSEIN SAMADI KAFIL2, NASER HARZANDI1, SEYYED REZA MOADDAB3*
1Department of Microbiology, College of Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran - 2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran - 3Department of Laboratory Sciences, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran
Water is one of the important reservoirs of environmental mycobacteria (EM). The resistance against chlorine and biofilm formation is among the important factors involved in colonization of these bacteria in water. Environmental mycobacteria cause different types of diseases especially pulmonary diseases due to their transfer to humans. The isolation and identification of these bacteria and the knowledge of how species are spread out as well as the identification of the dominant species in different geographical regions are necessary to be paid attention to because of differences in strategies for treating diseases caused by them. Different methods for isolating environmental mycobacteria have been employed by different researchers, but no standard method has been suggested for this isolation yet. Therefore, in the present study, in addition to introducing an ideal protocol, other studies related to the isolation of these bacteria from water resources were investigated, among which three top methods were selected and introduced. In addition, effective factors such as the type and concentration of disinfectants, the type of water processing, the type of culture medium, their pH, and incubation temperature involved in isolating bacteria are mentioned. Conclusion: using the filtration method instead of centrifuge one, using disinfectant of the cetylpyridinium chloride (CPC) instead of other disinfectants, using the Löwenstein-Jensen culture medium with an acidic pH, incubation in temperature of 30° C and incubation duration are among factors affecting in the isolation of environmental mycobacteria from the water sample.
isolation, environmental mycobacteria, drinking water