EMEL UZUNOGLUA, SAHIN DIREKELA, MERVE KOCBIYIKB, SELMA KELES ULUDAGC, AYSEGUL COPUR CICEKD
aAssistant Prof, Giresun University, Faculty of Medicine, Department of Medical Microbiology, Giresun, Turkey - bMsc, Recep Tayyip Erdoğan University Faculty of Arts & Sciences, Department of Biology, Rize, Turkey - cMD, Giresun Prof. Dr. A. Ilhan Ozdemir State Hospital, Medical Microbiology Laboratory, Giresun, Turkey - dAssociate Prof, Recep Tayyip Erdogan University, Faculty of Medicine, Department of Medical Microbiology, Rize, Turkey
Introduction: Carbapenem resistant Acinetobacter baumannii (A. baumannii) strains are challenging topics for hospitals. We determined the antibiotic susceptibilities and genetic resistance mechanisms of 135 A. baumannii isolates from Giresun State Hospital, Turkey between January 2013 and September 2014.
Material and methods: Antimicrobial susceptibility tests were performed according to the Clinical and Laboratory Standarts Institute guidelines. β-lactamase coding genes were investigated by simplex/multiplex PCR.
Results: High rates of multi drug resistance (51.11%) and extensively drug-resistance (48.14%) were remarkable. Colistin was seemed to be the only active compound against all clinical strains. Isolates were found 100% positive for blaOXA-51 and 95.55% posi- tive for blaOXA-23. Of all the isolates, 97.05% were found to be blaTEM (n=131) positive concomitant with whether blaOXA-51 or blaOXA23 or both them. All strains were negative for the rest of the β-lactamase coding genes. ISAb1 element was positive in the 98.51% (n=133) of the isolates and 100% of them were located upstream of blaOXA-51/23.
Conclusion: To our knowledge this study revealed the highest co-existence of blaOXA-51/23 and also demonstrated the increase of co-existence of both blaOXA-51/23 and ISAba1/blaOXA-51/23 over time in Turkey. The increasing combination of these genes and element may lead more resistance against to carbapenems among A. baumannii isolates.
Acinetobacter baumannii, ISAb1; blaOXA-51 or blaOXA23, Carbapenemase.