RAFIKA CHAIBI, SAWSEN DRINE, ALI FERCHICHI
Laboratory of Arid Land and Oases Cropping, Institute of Arid Regions, Médenine, Tunisia
Objectives: In this study we attempted to determine chemical composition, antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of extracts with different polarities (hexane, chloroform and methanol) obtained from Lawsonia inermis seeds and compared to those in controls.
Methods: Seeds of Lawsonia inermis were extracted successively in a Soxhlet system. The quantification of total phenolics of each extract was determined by the Folin-Ciocalteu method. The antioxidant scavenging activity was studied using 1, 1-diphenyl-2- picrylhydrazyl free radical (DPPH)/2,20-azinobis-3-ethylbenzothiazoline-6-sulphonate (ABTS) assays. Ascorbic acid was used as a standard. The anti-inflammatory activity of Lawsonia inermis seeds was determined on Soybean lipoxygenase. Cholinesterase (ChE) inhibitory activities were measured using Galanthamine as a positive control. The Cytotoxicity of each extract was estimated on human breast cancer cells (HTC-116) by the MTT (3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay.
Conclusion: The methanolic seed extract from Lawsonia inermis presented a very good antioxidant activity, a stronger 5- lipoxygenase and moderate acetylcholinesterase (AChE) activities. The strongest activity of Henna seed against human breast cancer cells (HTC-116) were obtained for the chloroform extract (IC50 = 45 mg/L). Our findings suggested that Lawsonia inermis seed could be a potential source of active molecule.
Lawsonia inermis (L), antioxidant, cytotoxic, cholinesterase, anti-inflammatory, phenolics