MEHRABANI NATANZI M1, KHODAII Z1, GERAYESH-NEJAD S2, KAMALINEJAD M3, SHARIFI R2, TAVANGAR SM4, DEH- POUR AR5, PASALAR P2*
1Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran - 2Department of biochemistry, School of Medicine, Tehran University of medical sciences, Tehran, Iran - 3Department of Pharmocognosy and Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran - 4Department of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran - 5Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Wound healing properties of Elaeagnus angustifolia (EA) fruit has been used for a long time in traditional Iranian medicine. The present study was performed to investigate the biochemical mechanism of wound healing activity of this plant in rats. After creating full-thickness skin wounds on the back of the rats, they were randomly divided into three groups. Two groups as the treated and positi- ve control received the extract of EA and mupirocin ointment, respectively and other group as the control group, was not subjected to any treatments. Isolated tissues from wounds were harvested for 5, 10, and 15 days and were then underwent biochemical analysis. Healing activity was assessed by the rate of wound contraction and collagen content. The in vivo antioxidant and anti-inflammatory activity was investigated to understand the mechanism of wound healing potency. The concentration levels of transforming growth fac- tor beta 1 (TGF-β1) and tumor necrosis factor alpha (TNF-α) were determined in wound tissues by an enzyme-linked immunosorbent assay (ELISA) and a real-time RT-PCR. The results indicated a significant increase in the percentage of wound contraction and colla- gen content in the treated group, when compared with the control and positive control groups. Both mRNA and protein levels of the TNF-α were significantly reduced in the EA treated group. A significant decrease was also observed in Malondialdehyde and Myeloperoxidase content in the treated group compared to the control group. This study revealed that the EA extract accelerates cuta- neous wound healing. This effect may confer its antioxidant and anti inflammatory activities. Therefore, EA extract may be useful in the current management of wound healing.ns.
Wound healing, Elaeagnus angustifolia, TNF-α, Antioxidant, Anti-inflammatory.