ZOHREH KHODAII1, 2, SAYYED MOHAMMAD HOSSEIN GHADERIAN3, MAHBOOBEH MEHRABANI NATANZI1, 2,*
1Dietary supplements and Probiotics research center, Alborz University of Medical Sciences, Karaj, Iran - 2Department of Biochemistry, Genetic and Nutrition, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran - 3Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Background and aim: Streptococcus mutans (S. mutans) is a Gram-positive, bacterium commonly found in the human oral cavity. S. mutans is a significant contributor to tooth decay. The potential application of probiotic bacteria for control and prevention of oral diseases has been the focus of the attention for various health researchers.
Material and methods: In this study, primarily, we examined the antibacterial activity of Lactobacillus routeri (L. routeri) against S. mutans using the spot agar test. Secondly, the ability of L. routeri to inhibit the adhesion of pathogenic S. mutans to human epidermoid laryngeal (HEp-2) cell line was tested using coincubation assay. In co incubation experiment, both bacteria were added to HEp-2 cells simultaneously and the number of adhered pathogen was counted in the presence and absent of probiotic bacteria.
Results: The result showed that, the presence of L. routeri decreased S. mutans adhesion by 4-folds.
Conclusion: In conclusion, probiotic bacteria may prevent colonization of oral cavity by odours producing bacteria: S. mutans.
Streptococcus mutans, Lactobacillus routeri, probiotic bacteria, HEp-2 cell.