Department of Medical Microbiology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey 


Introduction: Staphylococcus aureus is the etiological factor of one of the most widespread infections. This study aimed to inve- stigate the relationship between antibiotic resistance and the presence of Panton-Valentine leucocidin (PVL) in S. aureus strains.

Materials and methods: The methicillin resistance of the various strains was measured using a cefoxitin disc diffusion test. Presence of the ermA, ermC, mecA, tetK, tetM, gyrA, parC, aacA, and vanA genes and PVL (lukF/lukS) was investigated using the hybridization method following a multiplex polymerase chain reaction (PCR).

Results: In this study, 63.5% of the 96 strains were identified as methicillin-sensitive S. aureus (MSSA) and 36.5% as methicil- lin-resistant S. aureus (MRSA). In 15.6% of the S. aureus strains, only ermA was detected; in 10.4%, only ermC; in 2.1%, both ermA and ermC; in 36.5%, only mecA; in 11.5%, only tetK; in 18.8%, only tetM; in 1.0%, both tetK and M; in 1.0%, only the gyrA mutation; in 7.3%, only the parC mutation; in 14.6%, both gyrA and parC mutations; in 31.3%, aacA; in 5.2%, the PVL gene. The VanA gene was not detected in any strain.

Conclusion: According to our analysis, there may be S. aureus strains that carry but do not express known resistance genes, so other genes that may be responsible for resistance should be researched. 


S. aureus, MRSA, Resistance genes, MecA, PVL.