Department of Thoracic and Cardiovascular Surgery, Nantong First People’s Hospital, Nantong 226001, Jiangsu Province, China 


Objectives: Esophageal squamous cell carcinoma (ESCC) is associated with abnormal expression of multiple genes. Neuroepithelial transforming gene 1 (NET1) is a carcinogenic gene, overexpressed in several cancers. The tumor suppressor gene CD82, which encodes the protein KAI1, is down regulated in cancers. Lymphatic vessel invasion (LVI) is closely associated with meta- stasis. We investigated the expression levels of NET1, KAI1/CD82, LVI and their correlation with clinicopathological factors in ESCC.

Methods: Immunohistochemistry and Western Blot were used to detect NET1, KAI1/CD82 expression levels in the para-carcino- ma tissue and ESCC. LVI detected by D2-40 immunohistochemical staining. The relationships between the protein expression levels, positive LVI and clinicopathologic data were analyzed. Eighty-five patients, who had a primary resection of esophageal cancer, were analyzed by univariate and multivariate logistic regression, and univariate and multivariate survival analysis.

Results: NET1 expression levels in cervical cancer were significantly higher than in the para-carcinoma tissue, (P<0.05). KAI1/CD82 expression was markedly lower in ESCC than in the para-carcinoma tissue (P<0.05). NET1 high expression and positive LVI were positively correlated while KAI1/CD82 expression was negatively correlated with invasion, lymph node metastasis, and clini- cal stage. They were all independent prognostic factors for lymph node metastasis. Kaplan-Meier analysis revealed that NET1 high expression and positive LVI was negatively correlated with overall survival (OS), while KAI1/CD82 expression was positively correla- ted with OS. Low KAI1/CD82 expression, high expression of NET1, positive LVI was associated with a poor prognosis in ESCC. Multivariate Cox regression analysis indicated that the positive LVI were independent predictors for OS in ESCC.

Conclusions: NET1 high expression, positive LVI and KAI1/CD82 negative expression were all independent prognostic factors for lymph node metastasis in ESCC. Positive LVI was also an independent prognostic factor for OS. Combined detection of these fac- tors may be of significant value in predicting the prognosis and metastasis in ESCC patients. 


esophageal squamous cell carcinoma, NET1, KAI1/CD82, LVI, Metastasis.