Introduction: diabetic osteoporosis (DOP), due to the function impairment of bone marrow mesenchymal stem cells (BMMSCs), is commonly seen. The purpose of this study was to identify the different expressed microRNA (miRNA) in DOP BMMSCs.
Materials and methods: In our study, we used diabetic rats as models to explore the role of miRNA in DOP BMMSCs. First, the osteoporosis conditions of diabetic rats were assessed, then their BMMSCs were cultured to make the experimental group. At the same time, BMMSCs taken from normal rats were cultured as the control group. In the final stage, differently expressed miRNA were singled out using deep sequencing technology.
Results: The result showed that 8 weeks after the rats were induced to diabetes by streptozotocin (STZ) intraperitoneal injec- tion, the density of their humerus was lower, their BV/TV (bone volume relative to tissue volume)was smaller and the trabecular spa- cing was greater than the wistar group. In addition, 10 pieces of miRNA were selected for their striking differences.
Conclusion: By the significant enrichment information in pathway, the Notch signal pathway could regulate the diabetic osteo- porosis BMMSCs. Our study has provided new ideas in terms of a further discussion of how miRNA works in DOP.
diabetic osteoporosis (DOP), bone marrow mesenchymal stem cells (BMMSCs), microRNA