Introduction: To systemically compare the characteristics of integrin β2 signaling pathways related gene expressions among acute myocardial infarction (AMI) patients, stable angina pectoris (SA) patients and healthy controls.
Materials and methods: 20 AMI patients, SA patients and healthy people were enrolled into AMI, SA and control groups respectively. Gene expressions associated with signaling pathways of integrin β2 of three groups were detected and compared by t test.
Results: Compared with controls, mRNA expressions of α/β subunit (ITGAD, ITGAL, ITGAM, ITGAX and ITGB2), ligands of integrin β2 (RAGE, JAM-1, Fibrinogen, ICAM-1, ICAM-3, ICAM-5 and uPAR), chemokine (fMLP and RAF), inside-out signaling pathway (RIAM, ADAP, SLP-76, PLCγ, RAPL, SPA1, α-actinin, calpain, Dok1, radixin, talin and 14-3-3ζ) and outside-in signaling pathway (Fgr, Hck, Lyn, PKC, c-ab1, Syk, Vav1/3, cdc42, mDia, Rac, RhoA and ROCK1/2) in AMI patients were significantly upre- gulated. However there was no significant difference of gene expressions of integrin β2 and its signaling pathway in SA patients com- pared to control group.
Conclusions: The inside-out signaling pathway was downregulated generally in AMI patients, but not in SA patients. However, the outside-in signaling pathway was activated to be upregulated in AMI patients, but not in SA patients. Thus, the activation of inte- grin β2 was irritated by external factors.
acute myocardial infarction, stable angina pectoris, integrin β2, mRNA gene