Introduction: In the course of time, many structural and functional changes occur at different levels, and they are all known to contribute towards aging. The purpose of the study was to determine the changes in the levels of folic acid, vitamin B12, free T3 and T4, TOS (total oxidant status) and TAS (total antioxidant status) as the rat ages from 6 to 36 months old.
Materials and methods: Sprague-Dawley rats were obtained, grown, and divided into five equal groups as follows: Group I (6 months old), Group II (12 months old), Group III (18 months old), Group IV (24 months old) and Group V (36 months old). The levels of folic acid, vitamin B12, free T3 and T4, total oxidant and antioxidant capacity in the blood were analyzed.
Results: Folic acid level in Group I was detected to be significantly higher compared to the other groups (P<0.001), and vita- min B12 levels in Group V were determined to be significantly lower than those of the other groups (P<0.001). Free T3 level in Group II and free T4 level in Group I were measured significantly higher compared to the other groups (P<0.005, P<0.01), and TAS level in Group V was determined to be significantly lower compared to Group I and II (P<0.05).
Conclusions: Changes in the levels of folic acid, vitamin B12, thyroid hormones, and total oxidant and antioxidant capacities, might contribute to the proper diagnosis and treatment procedures of age-related diseases.
Aging, antioxidant system, folic acid; free T3 and T4, vitamin B12